Tóm tắt
Management of hydrogen in the rumen is an important factor to be considered when developing strategies to control ruminant methane emissions since hydrogen can impair digestion and fermentation if it accumulates. The aims of this project were to analyse the effect of a methane inhibitor (bromochloromethane) on methane production, metabolic H flux and subsequent responses in SCFA production and rumen microbial community in small ruminants. It was hypothesized that the rumen microbiota would adapt to inhibition of methanogenesis and shift fermentation to reductive processes which would consume more reducing equivalents, but excessive H2 gas would still accumulate and impair fibre digestion. As predicted, the methane-inhibited rumen appeared to adapt to the high H2 levels by shifting fermentation to propionate which was mediated by an increase in the population of hydrogen-consuming Prevotella, Selenomonas and Porphyromonas spp. As the rumen adapted to the high H2 concentration the flow of metabolic hydrogen into SCFA increased by >20% but the majority of 2H (>80%) thường được tiêu thụ để hình thành khí mê-tan đã bị động link vào w88 mới nhất thải ra ngoài. Do đó, việc tiêu thụ lượng hydro dư thừa này để tạo ra chất nền cho động link vào w88 mới nhất sẽ cần phải cung cấp các chất bổ sung trong chế độ ăn uống để thúc đẩy sự hấp thu hydro hoặc tăng cường các con đường hydrootrophic nhỏ chẳng hạn như quá trình tạo acetogen khử tự dưỡng.
